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Sunday, March 22, 2020

The Effects of Temperature and Relative Humidity on the Viability of the SARS Coronavirus- COVID-19


since almost similar sa genetics tong SARS1 at SARS2 (aka Corona Virus COVID-19) If madaling mamatay tong SARS1 sa init,, Hopefully...sana.. PATAY KANG Ding SARS2 -CORONA VIRUS KA SA INIT SA PINAS!! lampas nga ng 40 deg C sa labas..
I OFF ANG MGA AIRCON!

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The Effects of Temperature and Relative Humidity on the Viability of the SARS Coronavirus

Academic Editor: Alain Kohl
Received25 Nov 2010
Revised31 Jul 2011
Accepted31 Jul 2011
Published01 Oct 2011

Abstract

The main route of transmission of SARS CoV infection is presumed to be respiratory droplets. However the virus is also detectable in other body fluids and excreta. The stability of the virus at different temperatures and relative humidity on smooth surfaces were studied. The dried virus on smooth surfaces retained its viability for over 5 days at temperatures of 22–25°C and relative humidity of 40–50%, that is, typical air-conditioned environments. However, virus viability was rapidly lost (>3 log10) at higher temperatures and higher relative humidity (e.g., 38°C, and relative humidity of >95%). The better stability of SARS coronavirus at low temperature and low humidity environment may facilitate its transmission in community in subtropical area (such as Hong Kong) during the spring and in air-conditioned environments. It may also explain why some Asian countries in tropical area (such as Malaysia, Indonesia or Thailand) with high temperature and high relative humidity environment did not have major community outbreaks of SARS.

1. Introduction

Severe acute respiratory syndrome (SARS), was a new emerging disease associated with severe pneumonia and spread to involve over 30 countries in 5 continents in 2003. A novel coronavirus was identified as its cause [13]. SARS had a dramatic impact on health care services and economies of affected countries, and the overall mortality rate was estimated to be 9%, but rising to 50% in those aged 60 or above [4]. A notable feature of this disease was its predilection for transmission in the health care setting and to close family and social contacts. The disease is presumed to be spread by droplets, close direct or indirect contact, but the relative importance of these routes of transmission is presently unclear. A study showed that viral aerosol generation by a patient with SARS was possible and therefore airborne droplet transmission was a possible means of transmission [5]. However, the role of fomites and environmental contamination in transmission of infection is presently still unclear. An outbreak of disease affecting over 300 residents in high-rise apartment block (Amoy Gardens) in Hong Kong could not be explained by respiratory droplet transmission from infected patients [6]. Infectious virus is detectable in the faeces [7], and aerosolization of virus in contaminated faeces is believed to be the mode of transmission of this outbreak [8].
We and others have reported that infectivity of SARS CoV (SARS coronavirus) was lost after heating at 56°C for 15 minutes but that it was stable for at least 2 days following drying on plastic. It was completely inactivated by common fixatives used in laboratory [910]. Another study showed that it was inactivated by ultraviolet light, alkaline (), or acidic () conditions [11]. Human coronaviruses have been shown to survive in PBS or culture medium with 5–10% FCS for several days [1214] but they only survive a few hours after drying [1314]. There have been some studies reporting an association between the SARS outbreak, metrological factors, and air pollution [1517]. Thus, information on the survival of the SARS coronavirus (SCoV) in the environment at different temperature and humidity conditions is of significant interest to understanding virus transmission. A recent study using surrogate coronaviruses (transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHC)) has investigated the effect of air temperature and relative humidity on coronavirus survival on surface [18]. The survival effects of these environmental factors on SARS coronavirus remain unclear. In the present study, we report the stability of the SARS coronavirus at different temperatures and relative humidity.

2. Material and Methods

2.1. Virus Strain and Cell Line
The SARS CoV strain used in this study is HKU39849. Foetal monkey kidney cells (FRhK-4) were cultured in minimal essential medium (MEM, Gibco, USA) with 10% foetal calf serum and penicillin streptomycin (Gibco, USA) at 37°C in 5% CO2 and were used for growing stock virus and for titration of viral infectivity [12].
2.2. Preparation of Stock Virus
Stock virus was harvested when infection approximately 75% of the cell monolayer of a virus infected flask manifested cytopathic effect (CPE). Infected cells were subjected to one cycle of freeze and thaw centrifuged at 2000 rpm for 20 minutes to remove cell debris and the culture supernatant was aliquoted and stored at −80°C until use.
2.3. Determination of Tissue Culture Infectious Dose (50%) (TCID50)
96-well microtitre plates containing 100 μL of confluent FRhK-4 were infected with 100 μL of serial 10-fold of dilutions of stock virus in minimal essential medium with 1% FCS (maintenance medium) starting from 10−1 to 108. Titrations were done in quadruplicate. Infected cells were incubated for 4 days at 37°C. Appearance of CPE was recorded daily. TCID50 was determined according to Reed and the Muench method [19].
2.4. Effect of Drying, Heat, and Relative Humidity
Ten microlitre of maintenance medium containing 107 TCID50 per mL of virus was placed in individual wells of a 24-well plastic plates and allowed to dry at room temperature (22~25°C) and relative humidity of 40–50% (i.e., conditions prevailing in a typical air-conditioned room). One hundred microlitre of MM was used to resuspend the virus at 0 hr, 3 hr, 7 hr, 11 hr, 13 hr, 24 hr, and up to 4 weeks and the residual virus infectivity was titrated. Controls in closed screw cap eppendorf tube were included each time and treated similarly but without drying.
The experiment was repeated at different temperatures (38°C, 33°C, 28°C) and relative humidities (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr. A nebulizer under a controlled condition was used to generate high and relative low humidity environment. All the experiments above were conducted in duplicate and the residual viral infectivity was titrated.
2.5. Infectivity Assay
The infectivity of residual virus was titrated in quadruplicate on 96-well microtitre plates containing 100 μL of confluent FRhK-4 cells. 100 μL of serial 10-fold of dilutions of virus in maintenance medium starting from 10−1 to 108 was added into FRhK-4 cells. The infected cells were incubated at 37°C for 4 days. Appearance of CPE was recorded daily. TCID50 was determined according to the Reed and Muench method [19].

3. Results

Ten microlitre of 107 TCID50 per mL of virus was placed in individual wells of a 24-well plastic plate (representing a nonporous surface) and dried. The dried virus was then incubated at different temperatures (38°C, 33°C, 28°C) at different relative humidity (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr and the residual viral infectivity was titrated. A similar experiment was conducted at room temperature and relative humidity of about 40–50% (air-conditioned room) for up to 4 weeks. Virus dried on plastic retained viability for up to 5 days at 22~25°C at relative humidity of 40~50% with only  loss of titre (Figure 1). After that virus infectivity is gradually lost ever time. Loss of virus infectivity in solution was generally similar to dried virus under these environmental conditions. This indicates that SARS CoV is a stable virus that may potentially be transmitted by indirect contact or fomites, especially in air-conditioned environments.
High relative humidity (>95%) at comparatively low temperature (28°C and 33°C) did not affect the virus infectivity significantly (Figure 2(a)). High temperature (38°C) at 80–90% relative humidity led to a 0.25~2 loss of titre at 24 hr (Figure 2(b)). However, if the dried virus was stored at high temperature (38°C) and high relative humidity (>95%), there was a further ~1.5  loss of titre for each time point up to 24 hr (0.38~3.38 ) when compared with high temperature (38°C) at a lower relative humidity 80–90% (Figures 3(a)–3(c)).

4. Discussion

Viruses do not replicate outside living cell but infectious virus may persist on contaminated environmental surfaces and the duration of persistence of viable virus is affected markedly by temperature and humidity. Contaminated surfaces are known to be significant vectors in the transmission of infections in the hospital setting as well as the community. The role of fomites in the transmission of RSV has been clearly demonstrated [20]. Survival of viruses on a variety of fomites has been studied for influenza viruses, paramyxoviruses, poxviruses, and retroviruses [21]. The human coronavirus associated with the common cold was reported to remain viable only for 3 hours on environmental surfaces after drying, although it remains viable for many days in liquid suspension [13]. Parainfluenza and RSV viruses were viable after drying on surfaces for 2 and 6 hours, respectively [2022]. In aerosolised form, human coronavirus 229E is generally less stable in high humidity [12]. The environmental stability of SCoV was previously unknown and this information is clearly important for understanding the mechanisms of transmission of this virus in a hospital and community setting.
In the present study, we have demonstrated that SARS CoV can survive at least two weeks after drying at temperature and humidity conditions found in an air-conditioned environment. The virus is stable for 3 weeks at room temperature in a liquid environment but it is easily killed by heat at 56°C for 15 minutes [9]. This indicates that SARS CoV is a stable virus that may potentially be transmitted by indirect contact or fomites. These results may indicate that contaminated surfaces may play a major role in transmission of infection in the hospital and the community.
Our studies indicate that SCoV is relatively more stable than the human coronaviruses 229E or OC43 and some other viral respiratory pathogens such as respiratory syncytial virus. These findings suggest that, while direct droplet transmission is an important route of transmission [23], the role of fomites and environmental contamination in virus transmission may play a significant role in virus transmission. In particular, fomites may contribute to the continued transmission of infection in the nosocomial setting that continues to occur in spite of the great attention and stringent precautions taken to prevent droplet spread. In addition to droplet precautions, reenforcing contact precautions and hand washing is called for.
Faecal contamination of SCoV coronavirus may thus be an effective route of transmission of the disease. The outbreak in Amoy Garden in Hong Kong which affected over 300 residents in a single-apartment block with thought to have been transmitted by contaminated sewage. The stability of the virus on environmental surfaces and its presence in faeces indicates the potential that fecal contamination of fresh-food production may pose a threat for virus transmission; especially in countries with poor sanitation and sewage disposal systems and that studies to address this possibility are needed.
In this study, we showed that high temperature at high relative humidity has a synergistic effect on inactivation of SARS CoV viability while lower temperatures and low humidity support prolonged survival of virus on contaminated surfaces. The environmental conditions of countries such as Malaysia, Indonesia, and Thailand are thus not conducive to the prolonged survival of the virus. In countries such as Singapore and Hong Kong where there is a intensive use of air-conditioning, transmission largely occurred in well-air-conditioned environments such as hospitals or hotels. Further, a separate study has shown that during the epidemic, the risk of increased daily incidence of SARS was 18.18-fold higher in days with a lower air temperature than in days with a higher temperature in Hong Kong [24] and other regions [1517]. Taken together, these observations may explain why some Asian countries in tropical area (with high temperature at high relative humidity) such as Malaysia, Indonesia, and Thailand did not have nosocomial outbreaks of SARS (Tables 1 and 2(a)–2(c)). It may also explain why Singapore, which is also in tropical area (Table 2(d)), had most of its SARS outbreaks in hospitals (air-conditioned environment). Interestingly, during the outbreak of SARS in Guangzhou, clinicians kept the windows of patient rooms open and well ventilated and these may well have reduced virus survival and this reduced nosocomial transmission. SARS CoV can retain its infectivity up to 2 weeks at low temperature and low humidity environment, which might facilitate the virus transmission in community as in Hong Kong which locates in subtropical area (Table 2(e)). Other environmental factors including wind velocity, daily sunlight, and air pressure, had shown to be associated with SARS epidemic, should also be considered [1617]. The dynamics of SARS epidemic involves multiple factors including physical property of virus, outdoor and indoor environments, hygiene, space, and genetic predispositions [1015172425]. Understanding the stability of viruses in different temperature and humidity conditions is important in understanding transmission of novel infectious agent including that of the recent influenza Apandemic H1N12009.

AreasTotalMedan ageDeathsCase fatality Ratio (%)No. of imported Cases (%)No. of HCW (%)First caseLast case

China5327NKn3497NA1002 (19)Nov-02Jun-03
Hong Kong17554029917NA386 (22)Feb-03May-03
Taiwan34642371121 (6)68 (20)Feb-03Jun-03
Singapore2383533148 (3)97 (41)Feb-03May-03
Viet Nam6343581 (2)36 (57)Feb-03Apr-03
Indonesia256002 (100)0 (0)Apr-03Apr-03
Malaysia5302405 (100)0 (0)Mar-03Apr-03
Thailand9422229 (100)1 (11)Mar-03May-03
Philippines14412147 (50)4 (29)Feb-03May-03

Total8096
7749.61421706 (21)



MonthAverage sunlight (hours)TemperatureDiscomfort from heat and humidityRelative humidity
MinMaxampm

(a) Kuala Lumpur, Malaysia
Jan62232High9760
Feb72233High9760
March72333High9758
April62333High9763
May62333High9766
June72233High9663
July72332High9563
Aug62332High9662
Sept62332High9664
Oct52332High9665
Nov52332High9766
Dec52232High9761

(b) Jakarta, Indonesia
Jan52329High9575
Feb52329High9575
March62330High9473
April72431High9471
May72431High9469
June72331High9367
July72331High9264
Aug82331High9061
Sept82331High9062
Oct72331High9064
Nov62330High9268
Dec52329High9271

(c) Bangkok, Thailand
Jan92032High9153
Feb82233High9255
March92434High9256
April82535Extreme9058
May82534Extreme9164
June62433Extreme9067
July52432High9166
Aug52432High9266
Sept52432High9470
Oct62431High9370
Nov82231High9265
Dec92031High9156

(d) Singapore
Jan52330High8278
Feb72331High7771
March62431High7670
April62431High7774
May62432Extreme7973
June62431High7973
July62431High7972
Aug62431High7872
Sept52431High7972
Oct52331High7872
Nov52331High7975
Dec42331High8278

(e) Hong Kong
Jan513187766
Feb413178273
March316198474
April41924Medium8777
May52328Medium8778
June52629High8677
July82631High8777
Aug62631High8777
Sept62529High8372
Oct72327Medium7563
Nov71823Moderate7360
Dec615207463

*Data is available at BBC weather website (http://www.bbc.co.uk/weather/world/city_guides/results).

Conflict of Interests

The authors declare that there is no conflict of interest.

References

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Copyright © 2011 K. H. Chan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Saturday, March 21, 2020

MARCH 21, 2020

 



Crisis or no crisis, NEVER in my entire life that we overpriced an item or hoard an item.. I am maybe a womanizer, & alcoholic, but NEVER a Profiteer..Our shop Lagunastar Industries always aim to sell at the lowest price possible..BAD TRIP sa mga kacomptitensya naming shop sa Bambang that overprice & hoard facemask & thermal scanners.....

 But it is IMPOSSIBLE to sell now a thermal scanner at Php1,000 each because the manufacturers' price of the infrared thermometers in China Tripled or even Quadrupled or even 5X higher when COVID-19 breaks out because they have like 1.4 Billion people to monitor & they can sell the infrared thermometers to other clients in Europe at much higher price, thus limiting the supply available to us..we have to be realistic..#thermalscanner #infraredthermometer

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Photos taken from the https://soldering102.blogspot.com 

Thursday, March 19, 2020

march 19, 2020

Working hard.. to deliver fast those good damn accurate forehead thermometer that we have to serve to our valued clients.. seeing those inaccurate thermal scanners at SM, Robinsons, & many others make me sick.. as what is the purpose of that scanning at checkpoint if your god damn thermometers are inaccurate & for machine temp? & the reading is 33deg C? it's just an added stress to the people making their immune system low making them vulnerable to virus.. so it is like you need to add 3.5 Deg C to get the TRUE READING.. so meaning, using those inaccurate or industrial thermometers, if the guard's or police reading is 36.1 deg C, then they will let it pass, since the threshold should be 38.0 deg C, but in reality, using inaccurate thermometers, then 36.1 +3 = the actual body temp of that person is like 39 deg C, then that person has high fever already! hays

 but i have to understand the fatigue of those guards/police/military manning the entrance/checkpoint , imagine if you are the one doing that.. so let us not give them added stress by complaining.. also if i am also them, i will just check & let go..even if i know that my thermometer is not doing really well. i also understand if the thermometer they are using gets defective readings in the end, because imaginge if you are the thermometer, even if you are just a machine, if you are used for a long period of time, sooner or later, you will be subjected to wear & tear..
#thermalscanner

Monday, March 16, 2020

March 16, 2020

To the girl i love: crisis now..so pra akong facemask..limited supply lang ako..so if kung gusto moko talaga, huwag na tayo maglokohan, please take me! kunin mo na ko at wag ka na pabebe pa.. or else somebody will get me..mawawalan ka..ikaw din..konting konti nalang kaming pogi na mayaman na mabait na hindi babaero na single na natitira! #facemask #thermometer #soldering #memalang #N95 #coronavirus

Friday, March 13, 2020

March 13, 2020

I'm in a dilemma now, we can ship fast some high priced face mask from China & Europe like the KN95 & other high end facemask,as it is the only ones that is with stocks & can be shipped fast via Air..Those low end disposable masks can only be shipped via sea, so they are slow to ship. But if we will sell those higher end face masks that is gold in price now in China, we might be accused of overpricing..Because ang mahal talaga..Never in our entire life that we overpriced an item..yes, negosyante po ako,pero mas hilig ko po ang magkajowa kesa sa magkapera..#soldering #facemask

Thursday, March 12, 2020

March 12, 2020

I formally surrender & concede defeat in my latest romantic quest.

To you my love- The last 2 years has been a roller coaster in my life. I gave it all that i have..but i guess finding true love is my curse.. Demands of my work as a salesman greatly affects my performance as a lover.. I am willing to climb so many mountains & drive long hours just to have a glimpse of you..You are so beautiful & gorgeous, &  I love you & I really hope for your success..That is all i can say now..

To my competition & 3rd party- Ingatan mo siya, gumawa siya ng madaming FB account para lang sayo..hahaha

To my self- Move on, & to the next episode please.. & focus on the deliveries of Facemask , Thermal scanners & soldering soldering tools..

#memalang #wagpansinin #soldering #facemask #thermalscanner


Friday, March 6, 2020

MARCH 6, 2020

Masakit mabroken hearted..At nakakapanghinayang yung mga effort, oras, gasolina, toll , parking fee na naubos mo.. hahaha.. pero sa isang banda..if the love is not reciprocated or if the relationship is one sided only..para kang nakalaya..fresh beginning kumbaga..like breathing the fresh morning air..with full of hope of a new beginning of a new chapter in your life.. kasi kahit nga basura or scrap malaki ang value..depende sa tao na makakita sa yo..so humanap ka nalang talaga ng babaeng makakaappreciate sa real value mo.. #soldering #microscope

To Win Big...

"To win big, you sometimes have to take big risks."

- Bill Gates

Thursday, March 5, 2020

Ms. Loisa Andalio


photos taken from IG

@iamandalioloisa




Ms Bianca Umali at Preview Magazine 2020


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Perched on a makeup chair with her profile towards me, #BiancaUmali’s presence is undeniably intimidating, for lack of a better word. Perhaps it has something to do with her defined features: those large, piercing eyes, pouty lips, and a nose so naturally perfect that it had even become a point of discussion among gossipmongers. Such good looks have only served as the facade of what one can call the enigma of Bianca Umali. Read the full cover story in the link in our bio! #BiancaLovesPreview
Produced by Marj Ramos and Steph Sison⁠
Photographed by Charisma Lico⁠
Art Directed by Bacs Arcebal⁠
Co-produced by Jam Nitura⁠
Fashion Direction and Styling by Yanna Lopez⁠
Beauty Direction by Nicole Arcano⁠
Makeup by Rick Calderon⁠
Hair by Jan Edrosolan⁠
Words by Nicole Cruz
Special thanks to @funkyquarters in La Union!
Bianca wears a maillot from @wearsundae and sneakers from @converseph

Ms. Mika Salamanca- Crush ng Bayan!



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